Synthesis of silver-nisin nanoparticles with low cytotoxicity as antimicrobials against biofilm-forming pathogens

Wound infection is a serious threat to patients, in particular those with septic wound infections, which result high mortality rates. Moreover, the treatment of infections antimicrobial-resistant and/or biofilm-forming pathogens can be challenging. Nisin, potent antimicrobial against Gram-positive bacterial pathogens, has been used food industry as preservative for decades. Silver approved by FDA topical antimicrobial. Here, we show that silver-nisin nanoparticles (Ag-nisin NP), an average diameter 60 nm, quickly synthesized assistance simple microwave. Ag-nisin NP act bactericidal antibiotics tested pathogens. In contrast, resistance was observed S. aureus and A. baumannii were treated silver nitrate alone. addition, showed antibiofilm activity aureus, P. aeruginosa, baumannii, K. pneumoniae, E. coli, are occurring infections. Notably, lower cytotoxicity than human cells. This formulation provides alternative safe measurement biofilm-infected control.